PowerSoil DNA Isolation Kit適用于哪些珠磨研磨設(shè)備?
A. 渦旋儀
B. PowerLyzer
C. FastPrep
D. Precellys
E. 以上所有設(shè)備
答案是E!MO BIO的盒子可以用在所有珠磨研磨設(shè)備
今天討論一個(gè)非常棒的問(wèn)題!許多人問(wèn),我們的試劑盒適用于哪些珠磨研磨設(shè)備。事實(shí)上我們已經(jīng)把盒子的兼容性推到頂點(diǎn),它們能兼容市面上所有類(lèi)型的珠磨研磨設(shè)備。繼續(xù)往下看……
你好,
我對(duì)PowerSoil® DNA Isolation Kit很感興趣。我想配合FastPrep®使用,可以嗎?你們的Tube管能適用于這個(gè)機(jī)器嗎?
祝好,
博士研究生
我非常高興收到你提問(wèn)。我們有許多關(guān)于PowerSoil® DNA Isolation Kit配合FastPrep使用的文獻(xiàn)。本文章附文中列舉了近兩年的相關(guān)參考文獻(xiàn)。使用FastPrep去做PowerSoil的經(jīng)典設(shè)置是5或6檔45s一個(gè)循環(huán)。對(duì)于任何第一次接觸的土樣,我們一直建議經(jīng)過(guò)試驗(yàn)找出最佳的研磨方案 ,看什么條件下獲得的DNA完整性最好,得率最高。
在2010年我們發(fā)布了一款使用玻璃研磨珠的PowerSoil Kit。它的名字叫PowerLyzer PowerSoil DNA Isolation Kit。原來(lái)的PowerSoil Kit和新款PowerLyzer PowerSoil Kit采用的都是一樣的化學(xué)試劑盒操作方法流程。不同在于后者使用了0.1mm的玻璃研磨珠。試驗(yàn)發(fā)現(xiàn),玻璃研磨珠在高速珠磨研磨設(shè)備的強(qiáng)大作用力下不易破碎,非常適合裂解土壤中的微生物。從上面提到的"最佳研磨方案"文章中還能看到不同類(lèi)型土樣DNA得率增加的情況。
雖然兩種研磨珠都能用于強(qiáng)力珠磨研磨設(shè)備。但PowerLyzer PowerSoil Kit會(huì)更適合。
一個(gè)對(duì)使用普通渦旋儀與PowerLyzer提取6種不同類(lèi)型土樣做的對(duì)比實(shí)驗(yàn)見(jiàn)這里。其最終結(jié)論是,兩種研磨方法對(duì)提取效果的影響遠(yuǎn)不如土壤質(zhì)地、微生物含量重要。
最后,所有我們的DNA和RNA提取試劑盒提供的2ml研磨珠套管都能適用于所有類(lèi)型珠磨研磨設(shè)備。對(duì)我們供應(yīng)的研磨珠類(lèi)型以及用途詳細(xì)介紹見(jiàn)這里。
在MO BIO的實(shí)驗(yàn)室,我們專(zhuān)門(mén)研究部了如何開(kāi)門(mén)…當(dāng)然是細(xì)菌和真菌細(xì)胞壁!
如果你的樣品非常難裂解,需要尋求幫助以找到最好的破解辦法以及獲得最高的DNA得率,不妨聯(lián)系我們!
配合FastPrep使用PowerSoil Kit的相關(guān)參考文獻(xiàn):
- Shifts in Microbial Community Composition and Physiological Profiles across a Gradient of Induced Soil DegradationGuilherme M. Chaer, Marcelo F. Fernandes, David D. Myrold, and Peter J. Bottomley Soil Sci. Soc. Am. J., Jun 2009; 73: 1327 – 1334.
- Variations in Archaeal and Bacterial Diversity Associated with the Sulfate-Methane Transition Zone in Continental Margin Sediments (Santa Barbara Basin, California)Benjamin K. Harrison, Husen Zhang, Will Berelson, and Victoria J. OrphanAppl. Envir. Microbiol., Mar 2009; 75: 1487 – 1499.
- Diversity of Basidiomycetes in Michigan Agricultural SoilMichael D. J. Lynch and R. Greg ThornAppl. Envir. Microbiol., Nov 2006; 72: 7050 – 7056.
- Community Structure in the Sediment of a Freshwater Stream with Variable Seasonal FlowSteven A. Wakelin, Matt J. Colloff, and Rai S. KookanaAppl. Envir. Microbiol., May 2008; 74: 2659 – 2668.
- Changes in Bacterial and Archaeal Community Structure and Functional Diversity along a Geochemically Variable Soil ProfileColleen M. Hansel, Scott Fendorf, Phillip M. Jardine, and Christopher A. FrancisAppl. Envir. Microbiol., Mar 2008; 74: 1620 – 1633.
- Molecular Profiling of Rhizosphere Microbial Communities Associated with Healthy and Diseased Black Spruce (Picea mariana) Seedlings Grown in a NurseryM. Filion, R. C. Hamelin, L. Bernier, and M. St-ArnaudAppl. Envir. Microbiol., Jun 2004; 70: 3541 – 3551.
- Mycobacterium aviumsubsp. paratuberculosis in the Catchment Area and Water of the River Taff in South Wales, United Kingdom, and Its Potential Relationship to Clustering of Crohn's Disease Cases in the City of CardiffR. W. Pickup, G. Rhodes, S. Arnott, K. Sidi-Boumedine, T. J. Bull, A. Weightman, M. Hurley, and J. Hermon-TaylorAppl. Envir. Microbiol., Apr 2005; 71: 2130 – 2139.
- Molecular Fingerprinting of the Fecal Microbiota of Children Raised According to Different LifestylesJohan Dicksved, Helen Floistrup, Anna Bergstrom, Magnus Rosenquist, Goran Pershagen, Annika Scheynius, Stefan Roos, Johan S. Alm, Lars Engstrand, Charlotte Braun-Fahrlander, Erika von Mutius, and Janet K. Jansson Appl. Envir. Microbiol., Apr 2007; 73: 2284 – 2289.